DOT and non-DOT
DOT vs Non-DOT Drug Testing
DOT drug testing is federally regulated testing for safety-sensitive transportation jobs. It has to follow 49 CFR Part 40, and instant tests can't be used for it. Everything else is non-DOT testing, where an instant test gives a result on site in minutes.
The differences, side by side
| DOT testing | Non-DOT testing | |
|---|---|---|
| Who it covers | Safety-sensitive jobs regulated by FMCSA, FAA, FRA, FTA and PHMSA, and maritime jobs under the Coast Guard | Every other job, under the employer's own policy |
| Rules | 49 CFR Part 40 and the agency's own rule | Company policy and state law |
| Collection | A qualified collector, on the federal Custody and Control Form | As the policy sets: on site or at a collection center |
| Where the sample is tested | An HHS-certified laboratory | On site with an instant test, or at a laboratory |
| Drugs tested | The panel and cutoffs set in Part 40 | Chosen by the employer |
| Result review | A Medical Review Officer reviews every laboratory result that isn't negative | As the policy sets; confirm non-negative screens at a laboratory |
| Instant tests allowed | No | Yes, subject to state rules |
If your drivers or crews are DOT-regulated
Use a collection site that works with an HHS-certified laboratory and a Medical Review Officer, usually through a third-party administrator. We don't provide DOT testing.
For everyone else: non-DOT testing
Company-policy testing can use instant tests, subject to state rules. We sell 32 CLIA waived cups from $0.79 a cup at 20,000+, plus oral fluid tests and single-drug strips.
Workplace testing (non-DOT)CLIA waived cups for non-DOT programs
12 Panel Drug Test (120ml)Detects PCP, OXY, COC, METH, OPI & More CLIA Waived & FDA Approved, Serenity Click Cup- 50+
- $2.87
- 1,000+
- $2.39
- 20,000+
- $1.69
12 Panel Drug Test Cup, TCA, CLIA Waived and FDA approved- 50+
- $2.87
- 1,000+
- $2.39
- 20,000+
- $1.69
13 Panel Drug Test Detects Fentanyl 1ng: CLIA Waived & FDA Approved- 50+
- $2.97
- 1,000+
- $2.59
- 20,000+
- $1.79
14 Panel Drug Test Cup detects Fentanyl, CLIA Waived with EDDP & TCA – Clearance!- 50+
- $2.94
- 1,000+
- $2.45
- 20,000+
- $1.59
12 Panel Drug Test Cup 90ml: CLIA Waived, FDA Approved, Serenity Urine Test Cup- 50+
- $2.77
- 1,000+
- $2.19
- 20,000+
- $1.59
12 Panel Drug Test Detects TCA,OPI, OXY, METH, Adulterants, & More CLIA Waived- 50+
- $2.87
- 1,000+
- $2.39
- 20,000+
- $1.69
10 Panel Drug Test Cups AMP, MET, THC, MTD, OPI, OXY- 50+
- $2.65
- 1,000+
- $2.19
- 20,000+
- $1.59
- 15 Panel Drug Test Cup Detects Fentanyl, Nor Fentanyl & EDDP, CLIA Waived- Clearance!
- 50+
- $3.05
- 1,000+
- $2.49
- 20,000+
- $1.89
Questions about DOT and non-DOT testing
Can I use an instant drug test for DOT testing?
No. DOT-required tests must be collected on the federal Custody and Control Form and analyzed at an HHS-certified laboratory, with a Medical Review Officer reviewing results. Instant cups, dip cards and oral swabs can't be used for them.
What is a non-DOT drug test?
Any drug test that isn't required by a DOT agency: pre-employment, random, post-accident or reasonable-suspicion testing under a company's own policy. The employer chooses the drugs and the method, within state law.
What does a DOT drug test check for?
The drug classes and cutoffs set in 49 CFR Part 40. The rule changes from time to time, so check the current version or ask your laboratory or third-party administrator.
Where do DOT employers get tested?
Through a collection site that works with an HHS-certified laboratory and a Medical Review Officer, often arranged by a third-party administrator (TPA). We don't provide DOT testing.
Do non-DOT positive results need to be confirmed?
Yes. A positive result on an instant test is preliminary. Before any legal, employment or clinical action, have the specimen confirmed by a certified laboratory using GC-MS or LC-MS/MS. 12 Panel Now isn't affiliated with a specific laboratory, so customers arrange confirmation testing independently.